분자유전학실험실 (단국대학교 분자생물학과)



 이성욱 ( 2017-09-29 22:53:14 , Hit : 578
 Technique Adapted from CRISPR-Cas9 Corrects Mutation in Human Embryos

http://www.the-scientist.com/?articles.view/articleNo/50529/title/Technique-Adapted-from-CRISPR-Cas9-Corrects-Mutation-in-Human-Embryos/&utm_campaign=NEWSLETTER_TS_The-Scientist-Daily_2016&utm_source=hs_email&utm_medium=email&utm_content=56852840&_hsenc=p2ANqtz--_JHAi4B7vxG6XT5RTxptCi1GZwp8dJF8kUQlrwkAnwNs6EiQ9VofU3KQBKI-PwcjF3LgLoQ0v7zNHk2LIYtidPntvPg&_hsmi=56852840/

The Scientist »
The Nutshell

Researchers use base-editing to swap out an erroneous nucleotide responsible for a potentially life-threatening blood disorder.

By Catherine Offord | September 28, 2017

A team of Chinese researchers who were the first to report having applied CRISPR to human embryos have done it again—this time upgrading their technique to base-editing, a method of genome editing that corrects point mutations with higher efficiency than traditional CRISPR-Cas9 techniques. The scientists corrected a single nucleotide error responsible for β thalassemia, a potentially life-threatening blood disorder, according to a study published last week (September 23) in Protein and Cell.

Although the resulting embryos were mosaic—that is, they contained both corrected and uncorrected cells—the technique has the potential for higher precision than previous approaches.

See “Advances in Genome Editing”

“The paper itself represents a significant technical advance,” Darren Griffin, a geneticist at the University of Kent in the U.K., tells The Guardian. “Rather than using the classic Crispr technology previously reported, the current ‘base editor’ technology is an adaptation that chemically alters the DNA bases themselves.”

See “CRISPR: No Cutting Required”

Unlike older technologies, base-editing does not cleave the DNA when it makes an edit—a feature associated with fewer harmful side effects. To test the approach in humans, the Sun Yat-sen University researchers created cloned embryos using tissue from a patient with β thalassemia, which affects around 1 in 100,000 people worldwide, and has been the target of previous CRISPR-based attempts to eradicate disease-causing mutations.

See “CRISPR Corrects Blood Disorder”

The team then scanned the DNA in these embryos for the error—a G where there should have been an A—and converted it back using base-editing. Although the researchers acknowledge in their paper that further research is needed before the technique could be used therapeutically in humans, study coauthor Junjiu Huang tells BBC News that “we are the first to demonstrate the feasibility of curing genetic disease in human embryos by base editor system.”

The study’s publication comes just weeks after a US-Korean team reported using CRISPR-Cas9 to eradicate a defective mutation associated with a cardiac disease. That project was hailed as the first study to edit viable human embryos, but was soon met with criticism from other researchers who called the authors’ interpretation of the data into question.







  Technique Adapted from CRISPR-Cas9 Corrects Mutation in Human Embryos  이성욱 2017/09/29 578
1246   RNA-Based Gene Therapy Can Be Turned ON or OFF via Synbio Switches  이성욱 2018/10/18 580
1245   Frances H. Arnold, George P. Smith, and Gregory P. Winter share 2018 Nobel Prize in Chemistry  이성욱 2018/10/04 584
1244   FDA Approves First Gene Therapy for Inherited Disease  이성욱 2017/12/22 588
1243   Keytruda Gets FDA Approval for Hepatocellular Carcinoma  이성욱 2018/11/14 588
1242   Alcohol Boosts Cancer Risk by Damaging Stem Cell DNA  이성욱 2018/01/05 595
1241   Shields Up for New Cancer Gene-Therapy Virus  이성욱 2018/02/01 595
1240   Immunotherapy Pioneers Take 2018 Nobel Prize  이성욱 2018/10/02 603
1239   Checkpoint Inhibitor Generated by Synthetic DNA in Vivo  이성욱 2018/10/06 605
1238   Expanding the reach of gene editing with a new CRISPR enzyme  이성욱 2018/10/26 611
1237   CRISPR Enhancement Cures Genetic Disease in Mice  이성욱 2018/10/10 612
1236   [미국] 2017년, 유전자가위 기술을 통한 네 가지 사건들  이성욱 2018/01/10 613
1235   Novel Two-Pronged Method Targets Cancer Cells’ Telomerase and Chromosomes  이성욱 2018/11/03 613
1234   Second CRISPR-Modified Pregnancy May Be Underway  이성욱 2018/11/30 614
1233   Scientists Doubt Results of CRISPR’d Embryos  이성욱 2017/09/02 615
1232   Human Genome Editing Committee Urges Halt in Germline Editing Trials  이성욱 2018/11/30 619
1231   분자수준 인체 간세포 지도 처음 작성  이성욱 2018/10/24 625
1230   Nonviral CRISPR Delivery a Success  이성욱 2017/10/03 628
1229   DNA Repair Mechanism Aids Cancer by Mending Chemo Damage  이성욱 2017/11/10 640
1228   CRISPR Used in Human Embryos to Probe Gene Function  이성욱 2017/09/22 657

[1] 2 [3][4][5][6][7][8][9][10]..[64] [다음 10개]
 

Copyright 1999-2023 Zeroboard / skin by ROBIN