분자유전학실험실 (단국대학교 분자생물학과)



 이성욱 ( 2016-03-26 15:26:58 , Hit : 798
 CRISPR Can Tag RNA

The Scientist »
The Nutshell

Modifications to the DNA-slicing program allow for monitoring the movement of messenger RNA.

By Kerry Grens | March 21, 2016

WIKIMEDIA, ROBINSON R
CRISPR—a bacterial immune response best known for its genome-editing applications in the lab—has yet again been adapted for scientific purposes, this time to track RNA within cells. Researchers modified various components of CRISPR so that they could snap on to a specified messenger RNA (mRNA) within the nucleus, then enter the cytoplasm as a complex, where its fluorescent signal could be tracked.

“This work . . . sets the foundation for manipulation of the transcriptome in addition to the genome by CRISPR/Cas9,” the authors wrote in their report, published in Cell last week (March 17).

The alterations included neutering the Cas9 enzyme—which ordinarily cuts DNA—so it would grab the RNA target without cleaving it, and sticking on a fluorescent protein to allow for visual tracking. A guide RNA, along with the addition of an oligonucleotide sequence, sent the Cas9 RNA-ward.

“Just as CRISPR-Cas9 is making genetic engineering accessible to any scientist with access to basic equipment, RNA-targeted Cas9 may support countless other efforts for studying the role of RNA processing in disease or for identifying drugs that reverse defects in RNA processing,” study coauthor David Nelles of the University of California, San Diego, said in a press release.







1127   Mysterious Eukaryote Missing Mitochondria  이성욱 2016/05/17 851
1126   Genetically Engineered Pigs May Fly as Cancer Models  이성욱 2016/05/17 922
1125   Blame Daddy for Some Epigenetically Induced Birth Defects in Children  이성욱 2016/05/17 930
1124   RNA 세상: 태초에 RNA의 퓨린은 어떻게 생성되었나?  이성욱 2016/05/13 1398
1123   Three studies show that the virus can cause birth defects in mouse embryos.  이성욱 2016/05/13 1580
1122   ‘로렌조 오일’ 유전자 치료로 효과  이성욱 2016/05/09 848
1121   First Data from Anti-Aging Gene Therapy  이성욱 2016/04/28 866
1120   정밀의학의 끝: 개인화된 종양백신을 개발하는 과학자들  이성욱 2016/04/26 1058
1119   CRISPR Aids Identification of Sporadic Parkinson’s Mutation in GWAS  이성욱 2016/04/22 841
1118   바이오통신원 [바이오토픽] 이제는 염기편집 시대, Cas9를 변형하여 DNA 한 글자만 바꾼다.  이성욱 2016/04/21 1168
1117   Hitting HIV with CRISPR/Cas9 Can Arouse Resistance  이성욱 2016/04/10 865
1116   GSK Gets Positive CHMP Opinion For Strimvelis To Treat Rare Disease ADA-SCID  이성욱 2016/04/02 1033
  CRISPR Can Tag RNA  이성욱 2016/03/26 798
1114   미니멀세포(minimal cell)를 만든 크레이그 벤터  이성욱 2016/03/26 954
1113   Minimal Genome Created  이성욱 2016/03/26 831
1112   Using Gene Editing to Delete HIV from Human T Cells  이성욱 2016/03/23 774
1111   RNA May Surpass DNA in Precision Medicine  이성욱 2016/03/23 819
1110   Zika Update  이성욱 2016/03/15 754
1109   CRISPR/Cas9을 이용하여 PRRS 바이러스에 감염되지 않는 돼지를 개발하다  이성욱 2016/03/14 1203
1108   유전자 가위, 매머드 복원-안전한 백신의 시대 리본 자르다  이성욱 2016/03/14 987

[1][2][3][4][5][6][7] 8 [9][10]..[64] [다음 10개]
 

Copyright 1999-2021 Zeroboard / skin by ROBIN