I. RNA as Ribozyme
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II. RNA as Aptamer
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III. RNA as Allosteric T/S Ribozyme
III. RNA as Allosteric T/S Ribozyme
'Central dogma'¿¡ ÀÇÇϸé RNA moleculeÀº ´Ü¼øÈ÷ Á¤º¸ Àü´ÞÀÇ ¸Å°³Ã¼¿¡ Áö³ªÁö ¾Ê¾Ò´Ù.
ÇÏÁö¸¸ Hammerhead ribozyme, Group-1 intron µîÀÇ ¹ß°ßÀ¸·Î ÀÎÇØ RNA moleculeÀÌ protein enzyme°ú °°ÀÌ target cleavage ¶Ç´Â replacementµî?catalytic functionÀÌ ÀÖÀ½ÀÌ ¾Ë·ÁÁö°Ô µÇ¾ú´Ù. ¶ÇÇÑ SELEX¶ó´Â methodology¸¦ ÅëÇÏ¿© °³¹ßµÇ¾îÁö´Â RNA aptamer moleculeÀº small chemical¿¡¼ protein¿¡ À̸£±â±îÁö ´Ù¾çÇÑ target molecule¿¡ specificity¿Í affinity¸¦ °¡Áö°í bindingÇÔÀ¸·Î½á Áø´Ü ¹× Ä¡·áÁ¦·Î¼ antibody¸¦ ´ëüÇÒ ¼ö ÀÖ´Â Â÷¼¼´ë molecule·Î¼ °¢±¤¹Þ°í ÀÖ´Ù.
À̿Ͱ°ÀÌ ´Ù¾çÇÑ ±â´ÉµéÀ» °¡Áö°í ÀÖ´Â RNA moleculesÀÇ Æ¯¼ºÀ» °áÇÕÇÏ¿©, protein enzyme°ú °°ÀÌ ligand binding¿¡ ÀÇÇÏ¿© activity°¡ Á¶ÀýµÇ´Â regulatable RNA moleculeÀÇ °³¹ßµÇ°í ÀÖÀ¸¸ç, ¿©·¯ ¹æ¸é¿¡¼ ´Ù¾çÇÑ ¹æ¹ýµéÀ» ÅëÇÏ¿© ÀÌ¹Ì »ó´ç¼öÀÇ engineered regulatable RNA moleculesÀÌ Á¸ÀçÇÑ´Ù.
< Scott K. Silverman
RNA
2003,9,377~383>
Engineered regulatable RNA molecules »Ó¸¸ ¾Æ´Ï¶ó ÃÖ±Ù ¼¼Æ÷³» ATP, GTP, glycine°ú °°Àº metabolites¿ÍÀÇ interactionÀ» ÅëÇØ gene expression À» regulationÇÏ´Â? naturally existing regulatable RNA moleculesÀÎ riboswitch elementsµéÀÌ Á¡Â÷ ¹ß°ßµÇ¾î Áö°í ÀÖÀ¸¸ç, mechanism ¿¬±¸¸¦ ÅëÇØ ÀÌ·¯ÇÑ RNA elementµéÀÌ Á¤¸» protein moleculeµé°ú °°ÀÌ ligand¿¡ ÀÇÇØ allosterically regulationµÊÀÌ º¸°íµÇ°í ÀÖ´Ù.
ÀÌ·¯ÇÑ ¿¬±¸ °á°úµéÀ» Åä´ë·Î ¿ì¸® ½ÇÇè½Ç¿¡¼´Â SELEX ¹æ¹ýÀ» ÅëÇÏ¿© °³¹ßµÈ RNA aptamer¿Í ribozymeÀ» °áÇÕÇÏ¿© ligand binding¿¡ ÀÇÇÑ structural change¸¦ À¯¹ßÇÔÀ¸·Î½á activity°¡ ¡®ON' or 'OFF'µÇ´Â aptazyme(aptamer+ribozyme)À» °³¹ßÇϰíÀÖ´Ù.